Nanopore Sequencing Nanopore sequencing is a third-generation, long-read sequencing technology that determines DNA or RNA sequences by measuring changes in electrical current as single nucleic acid molecules pass through nanoscale pores embedded in a membrane. Unlike traditional sequencing methods, nanopore sequencing does not require PCR amplification and enables real-time, single-molecule analysis. This technology produces ultra-long reads, often exceeding hundreds of kilobases, allowing accurate detection of structural variants, repetitive regions, haplotypes, and epigenetic modifications such as DNA methylation. Nanopore sequencing is most prominently developed by Oxford Nanopore Technologies and is widely used in genomics , transcriptomics, metagenomics, pathogen surveillance, and clinical research. Nanopore Sequencing Long-Read Sequencing Third-Generation Sequencing Single-Molecule Sequencing Real-Time Sequencing Ultra-Long Reads Structural Variant Detection Epigenetic Profi...