Skip to main content

MicroRNAs in tumor cells

DNA logic nanomachine for the accurate identification of multiple microRNAs in tumor cells


The use of dynamic DNA logic circuits for disease diagnosis at the molecular level plays a considerable role in biomedical fields. Nevertheless, how to create programmable nanomachines based on molecular logical gates to accurately identify multiple biomarkers from tumor cells remains a pivotal challenge. Herein, we developed a DNA-based nanomachine for analyzing and imaging multiple microRNAs (miRNAs) in cancerous cells with a logical AND operation. The triangular prism design of DNA nanomachine improved its performance in living cell research with high stability and served as a modularized framework for toehold-mediated strand displacement reactions and catalytic hairpin assembly circuits.

The results suggested that the nanomachine could efficiently enter cells with great biocompatibility and rapidly recognize the correct biomolecules with high sensitivity. The well-designed DNA-logic gate nanomachine enabled accurate diagnosis on multiple miRNA patterns in different cell lines and differentiation of aberrant expression in target cells, which provided a novel possibility for intelligent disease diagnosis using smart nanomachines at the molecular level.

Chemicals and materials


The sequences of synthesized oligonucleotides are listed in Table S1, the reagents and materials in the experiments are described in the Supplementary Material.

DNA prism probe was prepared through adding equimolar nucleic acid strands of S1, S2, S3, H1-T, H2-T/H2-T-FQ, Capture-T, Trigger, Apt-1, Apt-2 and Apt-3 in 1 × TAE-Mg2+ buffer (40 mM Tris buffer, 1 mM EDTA, 12.5 mM MgAc2, pH 8.0) to the final concentration of 1 μM. The mixture was then subjected to a thermal annealing process (Table S2)

The working principle of self-assembled DNA nanomachines for simultaneous identification and imaging of multi-miRNAs is exhibited in Scheme 1. Initially, the 3D DNA nanomachine was one-pot self-assembled from ten nucleic acid strands, of which three long strands (S1, S2, S3) for assembling the triangular prism framework, two strands (Capture-T and Trigger) for synthesizing an “Anchor” domain to identify target miRNAs, two hairpin strands (H1-T and H2-T) as CHA substrate strands, and three.

In this work, DNA-logic gate nanomachines activated by multi-target miRNAs within cells were successfully designed using a DNA triangular prism as the nano-vehicle and the CHA cascade circuit as a signal amplifier. After internalization, DNA nanomachines made logical responses and the Trigger strand could be efficiently released via TMSD in the presence of miRNA-21, miRNA-155, and miRNA-10b simultaneously. Whereafter, CHA was activated by dissociative Trigger strand to generate amplified.

tumor microenvironment, cancer stem cells, metastasis, angiogenesis, immune evasion, oncogenes, tumor suppressor genes, apoptosis resistance, epithelial-mesenchymal transition, tumor heterogeneity, cell proliferation, hypoxia, tumor markers, neoplastic transformation, chemotherapy resistance, cell signaling pathways, tumorigenesis, genomic instability, cancer immunotherapy, tumor metabolism

#TumorCells, #CancerResearch, #Oncology, #Metastasis, #TumorMicroenvironment, #CancerStemCells, #TumorImmunology, #CancerTherapy, #ApoptosisResistance, #TumorMarkers, #Angiogenesis, #CellProliferation, #CancerGenetics, #EMT, #TumorHeterogeneity, #TumorMetabolism, #Chemoresistance, #PrecisionOncology, #CancerSignaling, #GenomicInstability

International Conference on Genetics and Genomics of Diseases

Visit: genetics-conferences.healthcarek.com

Award Nomination: genetics-conferences.healthcarek.com/award-nomination/?ecategory=Awards&rcategory=Awardee

Award registration: genetics-conferences.healthcarek.com/award-registration/

For Enquiries: contact@healthcarek.com

Get Connected Here
---------------------------------
---------------------------------
in.pinterest.com/Dorita0211
twitter.com/Dorita_02_11_
facebook.com/profile.php?id=61555903296992
instagram.com/p/C4ukfcOsK36
genetics-awards.blogspot.com/
youtube.com/@GeneticsHealthcare

Comments

Post a Comment

Popular posts from this blog

Fruitful innovation

Fruitful innovation: Transforming watermelon genetics with advanced base editors The development of new adenine base editors (ABE) and adenine-to-thymine/ guanine base editors (AKBE) is transforming watermelon genetic engineering. These innovative tools enable precise A:T-to-G and A:T-to-T base substitutions, allowing for targeted genetic modifications. The research highlights the efficiency of these editors in generating specific mutations, such as a flowerless phenotype in ClFT (Y84H) mutant plants. This advancement not only enhances the understanding of gene function but also significantly improves molecular breeding, paving the way for more efficient watermelon crop improvement. Traditional breeding methods for watermelon often face challenges in achieving desired genetic traits efficiently and accurately. While CRISPR/Cas9 has provided a powerful tool for genome editing, its precision and scope are sometimes limited. These limitations highlight the need for more advanced gene-e...
Post a Comment
Read more

Genetic factors with clinical trial stoppage

Genetic factors associated with reasons for clinical trial stoppage Many drug discovery projects are started but few progress fully through clinical trials to approval. Previous work has shown that human genetics support for the therapeutic hypothesis increases the chance of trial progression. Here, we applied natural language processing to classify the free-text reasons for 28,561 clinical trials that stopped before their endpoints were met. We then evaluated these classes in light of the underlying evidence for the therapeutic hypothesis and target properties. We found that trials are more likely to stop because of a lack of efficacy in the absence of strong genetic evidence from human populations or genetically modified animal models. Furthermore, certain trials are more likely to stop for safety reasons if the drug target gene is highly constrained in human populations and if the gene is broadly expressed across tissues. These results support the growing use of human genetics to ...
Post a Comment
Read more

Genetic Test

Genetic test eliminates progressive retinal atrophy in English shepherd dogs Researchers at the University of Cambridge recently published their findings in Genes after identifying the genetic mutation that is causing progressive retinal atrophy (PRA) in English shepherd dogs. PRA is a group of inherited diseases causing progressive degeneration of the light sensitive cells within the back of the eyes. When it comes to PRA in dogs, they are born with normal vision but by the age of 4-5 they go totally blind with no treatment. According to the release, by identifying the canines carrying this disease before they lose vision, this can be then used as a tool to guide breeding decisions to prevent the passing of the disease onto puppies.1 Historically, owners did not realize their dog had PRA until they were middle-aged, which means it could have been passed on to puppies if they had bred, making this a hard disease to control. “Once the dog’s eyesight starts to fail there’s no treatment ...
Post a Comment
Read more